Re: Antibody Lot Test Question

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From:"Peter A. Takes" <> (by way of histonet)
To:histonet <>
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Different mfrs might tell you different things for their products, but
mixing lots is generally not a good idea.  Age, concentration, usable titer,
purity, are all factors involved here.

Generally speaking, material from the same lot should be identical to what
you have used from that lot already, therefore, no additional testing should
be necessary.  However, if there are any doubts, check with the mfr.  The
main factor here is the age of the material, if it is an older but still
somewhat viable lot, it may have lost a bit of its 'punch' and may require a
lower titer.  Individual labs may also have their rules on how to approach
same lot material ordered at different times.

Hope this helps.

Peter A. Takes, Ph.D., RAC
Director, Clinical & Regulatory Affairs
Ph. 1-314-615-6964; Pager: 841-9351


> Histonetters,
>         A couple of immnu lot testing queries....
> -How do you feel about mixing primary antibody lots together when there
> isn't enough of the old lot.
>  Whether you say "Yay" or "Nay", please include your reasoning.
> -If a new antibody lot arrives in the lab and it has the same lot number
> as the current tested antibody, is it necessary to test it?  Again,
> please share your reasons for agreeing or disagreeing.
> -What DO you do with a drunken sailor?
>                                         Many thanks,
>                                         Sandosis
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