Re: Antibody Lot Test Question

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From:tylee <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"


Customers should be able to assume that vials of antibody that have the
same lot number will have the same performance upon receipt in the lab. If
the vendor is reliable, testing of the same lot number of freshly opened
antibody should not be necessary. Once the vials are opened (thawed,
aliquots made, or whatever...) it is a different story, so comparison of
what has been stored in the lab with a fresh vial may give different

Vendors may have different lot numbers for the same bulk batch of raw
material. Each dispensed group of tubes (even if they are from the same
batch) should have a unique traceable lot number.

My 2 cents worth.

Ty Lee

From: Cynthia Favara <>
To: <>;
Subject: RE: Antibody Lot Test Question
Date: Tuesday, January 19, 1999 8:17 AM

	My understanding of lot numbers is that they are  batch numbers.
Large volume that has been aliquot. Therefore I would not mix different lot
numbers. I would also not mix the different samples from the same lot. Each
aliquot has the potential of being subjected to detrimental treatment,
temperature would be an example. I would check a new aliquot of antibody
even with the same lot number for the same reason.
	I view lot numbers as a way of checking with the company if I
happens to have problems at the bench.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286

> ----------
> From:[]
> Sent: 	Friday, January 15, 1999 9:02 PM
> To:
> Subject: 	Antibody Lot Test Question
> Histonetters,
> 	A couple of immnu lot testing queries....
> -How do you feel about mixing primary antibody lots together when there
> isn't enough of the old lot.
>  Whether you say "Yay" or "Nay", please include your reasoning.
> -If a new antibody lot arrives in the lab and it has the same lot number
> as the current tested antibody, is it necessary to test it?  Again,
> please share your reasons for agreeing or disagreeing.
> -What DO you do with a drunken sailor?
> 					Many thanks,
> 					Sandosis
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