Carla,

Cellosolve is the trademark for mono- and diakyl ethers of ethylene glycol
and their derivatives.

I'm guessing you are doing somekind of staining for gram positive and gram
negative bacteria.    The type of Cellosolve you probably should be using
is ethylene glycol monoethyl ether.

If you are doing Gram, staining most of the histology textbooks have a
requiem of staining methods.  I always used the Modified Brown-Brenn Method
from Desni Sheehan's book.  It does require Cellosolve.  If you don't have
this reference, I would be more than happy to send you a copy of the
procedure.

Rande Kline HT (ASCP)
Technical Services
EM Science




Carla_Aiwohi@usgs.gov (Carla Aiwohi) on 01/20/99 04:16:38 PM

To:   Histonet@pathology.swmed.edu
cc:
Subject:




I have a protocol which calls for 1% tartrazine in cellusolve.  Can anyone
tell
me what cellusolve is?  Is there anything I can use instead of cellusolove.
Thanks in advance,
Carla Aiwohi
Western Fisheries Research Center