RE: VIP Processor

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I process approximately 600-700 specimen cassettes per day on 4 VIP 2000's.
This program works well for me, especially when the smaller biopsies are
intermixed with larger fatty specimens. Run time approx. - 10 hours, 30 min.
I also rotate all my solutions twice a week. The lowest possible temperature
for your paraffins is best for the biopsies.

Station 			Reagent			Time
Heat(C)		PV Cycle

1				10% NB formalin	90 min		37
ON
2				10% NB formalin	90 min		37
ON
3				 Penfix				30 min
37				ON
4				80% ethanol			40 min
37 				ON
5 				95% ethanol			40 min
37 				ON
6 				100% ethanol		40 min		37
ON
7				100% ethanol		40 min		37
ON
8				100% ethanol		40 min		37
ON
9				Xylene				45 min
37				ON
10				Xylene				45 min
37				ON
11				Paraffin				30
min		57				ON
12				Paraffin				30
min		57				ON
13				Paraffin				30
min		57				ON
14				Paraffin				30
min		57				ON


Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center


	----------
	From:  ALF569@aol.com [SMTP:ALF569@aol.com]
	Sent:  Wednesday, January 27, 1999 10:52 AM
	To:  histonet@pathology.swmed.edu
	Subject:  VIP Processor

	Hi everyone I am looking for a little help regarding my VIP
Processor.
	We had an MVP processor for years and recently bought a VIP
Processor. We have
	set
	up our program exactly as it is on the MVP processor and we are
having
	problems with the larger tissues being unfixed and the biopsies
being dry. We
	process approximately 150 blocks a day. I have thought about
changing the 70
	and 80% alcohol twice a week if need be. I was under the impression
that this
	processor was supposed to keep the solutions cleaner. Any ideas?




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