RE: VIP Processor
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From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
I process approximately 600-700 specimen cassettes per day on 4 VIP 2000's.
This program works well for me, especially when the smaller biopsies are
intermixed with larger fatty specimens. Run time approx. - 10 hours, 30 min.
I also rotate all my solutions twice a week. The lowest possible temperature
for your paraffins is best for the biopsies.
Station Reagent Time
Heat(C) PV Cycle
1 10% NB formalin 90 min 37
ON
2 10% NB formalin 90 min 37
ON
3 Penfix 30 min
37 ON
4 80% ethanol 40 min
37 ON
5 95% ethanol 40 min
37 ON
6 100% ethanol 40 min 37
ON
7 100% ethanol 40 min 37
ON
8 100% ethanol 40 min 37
ON
9 Xylene 45 min
37 ON
10 Xylene 45 min
37 ON
11 Paraffin 30
min 57 ON
12 Paraffin 30
min 57 ON
13 Paraffin 30
min 57 ON
14 Paraffin 30
min 57 ON
Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center
----------
From: ALF569@aol.com [SMTP:ALF569@aol.com]
Sent: Wednesday, January 27, 1999 10:52 AM
To: histonet@pathology.swmed.edu
Subject: VIP Processor
Hi everyone I am looking for a little help regarding my VIP
Processor.
We had an MVP processor for years and recently bought a VIP
Processor. We have
set
up our program exactly as it is on the MVP processor and we are
having
problems with the larger tissues being unfixed and the biopsies
being dry. We
process approximately 150 blocks a day. I have thought about
changing the 70
and 80% alcohol twice a week if need be. I was under the impression
that this
processor was supposed to keep the solutions cleaner. Any ideas?
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