RE: Questions about fixation and incubation

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From:Cynthia Favara <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

	I have a protocol for CD1a and FActor XIIIa in fromalin fixed
paraffin embedded human tissue. I would be hapy to forward them to you if
you like. Let me know.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286

> ----------
> From:[]
> Sent: 	Saturday, January 23, 1999 11:38 AM
> To:
> Subject: 	Questions about fixation and incubation
> I am a dermatologist at Mt Sinai Med Ctr in NYC.  I am workingon a project
> where i stained for cytokines in human skin, IL-4, IL-5, IFN gamma using
> formalin fixation, overnight incubation at room temp and it worked ok.  We
> are
> also staining for CD-4, CD-8, CD-30, CD-1a and Factor XIIIa. Path
> recommended
> we use acetone fixation and incubation overnight at 4 degrees cent. and
> results have not been so good.  I am not sure which fixation to use and
> whether to incubate at room temp or 4 deg.  Any recommendations will be
> greatly appreciated.
> Donald Rudikoff

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