RE: Questions about fixation and incubation
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From: | Cynthia Favara <cfavara@atlas.niaid.nih.gov> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Donald,
I have a protocol for CD1a and FActor XIIIa in fromalin fixed
paraffin embedded human tissue. I would be hapy to forward them to you if
you like. Let me know.
Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov
> ----------
> From: RNAhybrid@aol.com[SMTP:RNAhybrid@aol.com]
> Sent: Saturday, January 23, 1999 11:38 AM
> To: histonet@Pathology.swmed.edu
> Subject: Questions about fixation and incubation
>
> I am a dermatologist at Mt Sinai Med Ctr in NYC. I am workingon a project
> where i stained for cytokines in human skin, IL-4, IL-5, IFN gamma using
> formalin fixation, overnight incubation at room temp and it worked ok. We
> are
> also staining for CD-4, CD-8, CD-30, CD-1a and Factor XIIIa. Path
> recommended
> we use acetone fixation and incubation overnight at 4 degrees cent. and
> results have not been so good. I am not sure which fixation to use and
> whether to incubate at room temp or 4 deg. Any recommendations will be
> greatly appreciated.
> Donald Rudikoff
>
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