Donald,
	I have a protocol for CD1a and FActor XIIIa in fromalin fixed
paraffin embedded human tissue. I would be hapy to forward them to you if
you like. Let me know.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov



> ----------
> From: 	RNAhybrid@aol.com[SMTP:RNAhybrid@aol.com]
> Sent: 	Saturday, January 23, 1999 11:38 AM
> To: 	histonet@Pathology.swmed.edu
> Subject: 	Questions about fixation and incubation
>
> I am a dermatologist at Mt Sinai Med Ctr in NYC.  I am workingon a project
> where i stained for cytokines in human skin, IL-4, IL-5, IFN gamma using
> formalin fixation, overnight incubation at room temp and it worked ok.  We
> are
> also staining for CD-4, CD-8, CD-30, CD-1a and Factor XIIIa. Path
> recommended
> we use acetone fixation and incubation overnight at 4 degrees cent. and
> results have not been so good.  I am not sure which fixation to use and
> whether to incubate at room temp or 4 deg.  Any recommendations will be
> greatly appreciated.
> Donald Rudikoff
>