>Date: Mon, 25 Jan 1999 22:45:39 -0600 (CST)
>From: fetching@webtv.net (bonnie greer)
>Subject: RE: Lung Embedding Protocol
>To: mark.lewis@shandon.com
>Cc: histonet@pathology.swmed.edu, brittman@mail.db.uth.tmc.edu
>MIME-version: 1.0 (WebTV)
>
>Embedding is the actual casting/ places the tissue into a mold in the
>correct position. Filling the lung with fixative is
>perfusion..........Bonnie G. Greer ,histo lab St. Jude Childrens
>Hospital/   Memphis,TN
>

Bonnie,
 	I have to differ. I would say filling the lung with fixative is
simply immersion fixation. Perfusion fixation is via the vascular system.
	While I'm in the grouching mode, I don't think inflating the lung
with fixative is very physiological. I realise that in Histo/Pathology labs
this is probably the only route available but when compared to air
inflation and vascular perfusion the architecture of the alveoli looks a
wee bit different.
	Let the debate begin.
Ian.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk