Paula,

I wonder whether your problems may stem from the slides themselves. Try the
Poly-Prep slides or even better the Silane-Prep slides from Sigma. Both are
coated to promote tissue section adherence.

Dorit

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From:  Paula Wilder
Sent:  יום שלישי 19 ינואר 1999 04:00
To:  HistoNet@Pathology.swmed.edu
Subject:  IHC: ER/PR tissues washing off

Hello Histonet!

Been out of contact with the net for several months, so I hope this
query is not a recent duplicate.  For some reason, all of a sudden, our
ER and PR tissues have been washing off the slide after antigen
retrieval using a rice steamer.  We use charged slides.  We have tried
keeping the slides overnight in our incubator oven at 60 degrees.  We
have thought about incubating the slides in a 75-80 degree oven for a
shorter period of time, but have not done so yet.
We have also thought about trying poly-L-lysine slides.  Do you have any
thoughts?  Any information will be very greatly appreciated.  THANK
YOU!!!!


Paula Wilder
St. Joseph Medical Center
Towson, MD

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