RE: Antibody Lot Test Question

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From:Cynthia Favara <cfavara@atlas.niaid.nih.gov> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Sandosis,
	My understanding of lot numbers is that they are  batch numbers.
Large volume that has been aliquot. Therefore I would not mix different lot
numbers. I would also not mix the different samples from the same lot. Each
aliquot has the potential of being subjected to detrimental treatment,
temperature would be an example. I would check a new aliquot of antibody
even with the same lot number for the same reason.
	I view lot numbers as a way of checking with the company if I
happens to have problems at the bench.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov



> ----------
> From: 	sjrugby@juno.com[SMTP:sjrugby@juno.com]
> Sent: 	Friday, January 15, 1999 9:02 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	Antibody Lot Test Question
>
> Histonetters,
> 	A couple of immnu lot testing queries....
>
> -How do you feel about mixing primary antibody lots together when there
> isn't enough of the old lot.
>  Whether you say "Yay" or "Nay", please include your reasoning.
>
> -If a new antibody lot arrives in the lab and it has the same lot number
> as the current tested antibody, is it necessary to test it?  Again,
> please share your reasons for agreeing or disagreeing.
>
> -What DO you do with a drunken sailor?
>
> 					Many thanks,
> 					Sandosis
>
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