I am a dermatologist at Mt Sinai Med Ctr in NYC.  I am workingon a project
where i stained for cytokines in human skin, IL-4, IL-5, IFN gamma using
formalin fixation, overnight incubation at room temp and it worked ok.  We are
also staining for CD-4, CD-8, CD-30, CD-1a and Factor XIIIa. Path recommended
we use acetone fixation and incubation overnight at 4 degrees cent. and
results have not been so good.  I am not sure which fixation to use and
whether to incubate at room temp or 4 deg.  Any recommendations will be
greatly appreciated.
Donald Rudikoff