Lung embedding protocol

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From:Katri Tuomala <> (by way of histonet)
To:histonet <>
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Hi all!
We also have a gizmo set up for perfusing whole human lungs, similar to
Katie's in Michigan. Following protocol is ment to deal with open lung
biopsies to diagnose lung diseases:

Reference: Churg A: An inflation procedure for open lung biopsies.
           American Journal of Surgical Pathology 7:69-71, 1983.

The portion of the lung biopsy to be processed to paraffin is distended
with formalin using 25 gauge butterfly needle connected to a 5 ml
syringe filled with formalin. After distension the specimen is immersed
in formalin and allowed to fix at least 30 minutes. All the tissue is
then cut into blocks and submitted for histology paraffin processing.

I hope this is helpful for all interested. Katri.

Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada

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