Good evening Histonetters
I have been asked to deploy a metallic stent in a coronary artery then
process for plastic embedding. The specimen will then be cured, sawed in
half and polished to a clear fininsh and evaluated under a dissection
microscope. I found in Poysciences a JB-4 embedding kit. My question is
to you all is what dehydration and infiltration technique should I use?
I was thinking of using the processing schedule below:
1	50% ETOH		30 minutes
2	60% ETOH		30 minutes
3	70% ETOH		30 minutes
4	85% ETOH		30 minutes
5	95% ETOH 		30 minutes
6	100% ETOH		30 minutes
7	100% ETOH		30 minutes
8	100% ETOH		30 minutes
9	100% ETOH/JB-4	4 hours
10	JB-4			overnight
11	Embed
It looked intriguing because it is water soluable, clear and
Polysciences says it can be done quickly.
Any advice?
Thanks Gordon