IHC: ER/PR tissues washing off

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From:Paula Wilder <histo20@hotmail.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hello Histonet!

Been out of contact with the net for several months, so I hope this
query is not a recent duplicate.  For some reason, all of a sudden, our
ER and PR tissues have been washing off the slide after antigen
retrieval using a rice steamer.  We use charged slides.  We have tried
keeping the slides overnight in our incubator oven at 60 degrees.  We
have thought about incubating the slides in a 75-80 degree oven for a
shorter period of time, but have not done so yet.
We have also thought about trying poly-L-lysine slides.  Do you have any
thoughts?  Any information will be very greatly appreciated.  THANK

Paula Wilder
St. Joseph Medical Center
Towson, MD

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