Fixation and embedding of adult murine eyes
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From: | Carolyn Pressman <cpress@gsbs3.gs.uth.tmc.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
What is the best fixative to use for adult murine eyes? Does anyone have
any good protocals? I have found through trial and error that fixing in
neutral buffered formalin only works ok if I fix for 2 days. I embed in
parafin and then section at 7 um. Most of the architecture of the eye is
intact, but the lens always tears or has holes in it. Is there a way to
get around this problem? Is embedding in plastic a better way to do this?
Carolyn Pressman
MD Anderson Cancer Center
University of Texas--Houston
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