Fixation and embedding of adult murine eyes

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From:Carolyn Pressman <> (by way of histonet)
To:histonet <>
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What is the best fixative to use for adult murine eyes?  Does anyone have
any good protocals?  I have found through trial and error that fixing in
neutral buffered formalin only works ok if I fix for 2 days.  I embed in
parafin and then section at 7 um.  Most of the architecture of the eye is
intact, but the lens always tears or has holes in it.  Is there a way to
get around this problem?  Is embedding in plastic a better way to do this?
Carolyn Pressman
MD Anderson Cancer Center
University of Texas--Houston

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