Diastase/Amylase digestion

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From:bbracing <bbracing@silk.net> (by way of histonet)
To:histonet <histonet@magicnet.net>
Content-Type:text/plain; charset="us-ascii"

A little help is needed.
We used to get a very good diastase from BDH (diastase of malt) that worked
as good as, or better than "spitting" on the slides for glycogen digestion.
The last several bottles have not worked well.  We have also tried material
obtained from Fisher and Sigma  again with very poor results. We used to
use a 0.25% in sailine for 10 min.at room temp.  With the materials that we
have recieved lately, we have tried every combination of solutions from
0.25% up to 5% with times ranging from 5 min to 30 min, but with very poor
results, when compared to  the same control slide that we simply "spit on"
We have far too many slides that require digestion,  on a daily basis to
continue with the  good old "spit" technology.  Has any one out in
histoland got a good source of diastase that works as well as good old
"spit"   Many thanks in advance.
Kerry Beebe
Kelowna Gen Hospital
Kelowna B.C.
e-mail  bbracing@silk.net

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