Ag-Ab control

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From:"Connolly, Brett" <brett_connolly@merck.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Can someone please provide me with a protocol or reference for
pre-incubating an antibody with excess antigen to use as a control in IHC.
The Ab is an anti-peptide rabbit serum of unknown IgG conc. I have access to
purified antigen.
Thanks,
Brett

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Human Genetics
WP26A-3000
P.O. Box 4
Sumneytown Pike & Broad St.
West Point, PA 19486
ph.215-652-2501
FAX. 215-652-2075
email:brett_connolly@merck.com




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