Re: [Histonet] tissue does not sink in sucrose

From:Geoff McAuliffe



Hi Yeves:

    You may have some fat mixed in with the pancreas and fat, with at=20
specific gravity of less than 1.0, will not sink.

Geoff


Yves Heremans wrote:
> Dear Histonetters,
>
> I am cryoprotecting mouse pancreas tissue (after fixation with 
> formalin) in 30% sucrose in PBS. After overnight incubation in sucrose 
> (at 4C) some tissues sink, others do not. Is there a reason (and=20
> remedy) for this ?
>
> Thanks in advance,
>
> Yves Heremans
>
> Brussels Free University - VUB
> Diabetes Research Center
> Building D Level 2 Room D258
> Laarbeeklaan 103
> 1090 Brussels, Belgium
> Phone: +32 (0)2 477 44 73
> www.betacelltherapy.org
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************




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