Re: [Histonet] tissue does not sink in sucrose

From:"Emily Sours"



If your tissue is large, it might not sink in one night.  Leave it for
a few days.

Emily

On Jan 18, 2008 3:45 AM, Yves Heremans  wrote:
> Dear Histonetters,
>
> I am cryoprotecting mouse pancreas tissue (after fixation with
> formalin) in 30% sucrose in PBS. After overnight incubation in
> sucrose (at 4C) some tissues sink, others do not. Is there a reason
> (and remedy) for this ?
>
> Thanks in advance,
>
> Yves Heremans
>
> Brussels Free University - VUB
> Diabetes Research Center
> Building D Level 2 Room D258
> Laarbeeklaan 103
> 1090 Brussels, Belgium
> Phone: +32 (0)2 477 44 73
> www.betacelltherapy.org
>
>
>
>
>
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>



-- 
"Prosperity ripened the principle of decay...and, as soon as time or
accident and removed the artificial supports, the stupendous fabric
yielded to the pressure of its own weight...instead of inquiring why
the Roman Empire was destroyed we should rather be surprised that it
has subsisted for so long"
-Edward Gibbon, Decline and Fall of the Roman Empire

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