Re: [Histonet] Whole Mount Slides

From:"Vicki Gauch"



Jennifer,
 We routinely cut whole mount prostate slides.  We cut them on a Leica
microtome with no problem.  When we embed them, we use the bottom of a
Tissue Tek mega cassette on top of the wax filled metal mold - that
seems to work for us.  The block stays put in the chuck holder during
cutting as long as you trim the sides of the mega cassette so it is
paraffin free and clearance hasn't really been an issue.  If you need
more info, please feel free to e-mail me....

Vicki Gauch
AMCH
Albany. NY

>>> "Bull, Jennifer L." 
1/21/2008 3:21 PM >>>
I am looking for any information on Whole Mount Slides. Our
pathologists
would like to start doing them on Prostate and Bladder tissue. I have
researched and located 
a microtome attachment from Leica and SurgiPath carries oversized
cassettes and embedding molds. Does anyone have any information to
add?
Processing/cutting issues? Supplies? I would like to be as informed as
possible before taking the plunge............  Thank You in advance!
 
Jennifer Bull
Histology Supervisor
NW Pathology
Bellingham, WA
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