I can be found on Members Only webpage/NSH website and as of September,
retired. However the Movats pentachrome, rather than trichrome, was the
protocol from Sheehan and Hrapchak, Theory and Practice of Histotechnology,
2nd edition. I did this over 20 years ago, but not in recent years. I know
there are modified and shorter methods than the one I used, and I did it on
methyl methacrylate embedded bone, ground sections.
I did the protocol from Sheehan and Hrapchak as theirs was modified for
methyl methacrylate plastic embedded bone but do not have this on a computer
file. If you have the textbook, it will be there. I have seen messages
(in Archives) which dealt with finding reagents, in particular, Safran du
Gatinas or saffron which can be purchases in a grocery store. As for where
to buy some of the dyes, I would be outdated for sources since the stain has
not been done for many years. We did make our staining reagents in house,
but you may get lucky finding a staining kit with Poly Scientific or
Google Bryan Llewellyn's Stains File website for two Movats protocols which
should work very well for you, one that uses the saffron from a grocery
store. A great website filled with information. I believe that Histo
Logic also has a modified Movats publication.
Good luck on your staining.
--- Original Message -----
From: "Colleen Forster"
Sent: Friday, January 18, 2008 4:31 PM
Subject: [Histonet] Movats Trichrome
> Looking for Gayle Callis. Would you be willing to share your Movats
> Trichrome protocol and reagent list...including where you purchase them
> Thanks in advance,
> Colleen Forster
> U of MN
> Histonet mailing list
Histonet mailing list