Re: [Histonet] Decal Bone Antigen retrieval

From:"Gayle Callis"

Some people like waterbath, at just below boiling temperature. at 99C, 
preheat Citra buffer to working temperature while deparaffinizing sections, 
immerse sections for 10 min or longer, depending on protocol, remove then 
rinse slides with PBS, proceed with staining.  This was a method given to me 
some years ago by an expert on retrievals.  Very gentle to bone sections. 
You may have to optimize time for the antigen, and how you handle the 
section after the initial heating.

Gayle M. Callis
Bozeman MT 59715

----- Original Message ----- 
From: "Igor Deyneko" 
Sent: Tuesday, January 22, 2008 11:51 AM
Subject: [Histonet] Decal Bone Antigen retrieval

> Dear histonetters!
> I have a question. Recently I have received decaled mouse hind paw joint
> slides, which I have to do IHC on. Can anyone advise an antigen retrieval
> solution and a method. The current method I use and has been working fine 
> in
> the past is as follows:
> Either Citrate or DAKO Retrieval solution in a conventional pressure 
> cooker
> for 20 minutes , then I would let it stand with the open lid for another 
> 10
> minutes, and finally would take the Coplin jars out and let them stand for
> an additional 30 mins at RT. Worked fine for tumors. Any ideas for 
> joints???
> All suggestions would be greatly appreciated.
> Igor Deyneko
> Infinity Pharmaceuticals
> Cambridge, MA 02139
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