Re: [Histonet] Aqueous Mounting Medium in In-Situ

From:"Gayle Callis"


Since you are doing fluorescent ISH, I suggest using a hard set aqueous 
mounting media that helps prevent fading of the fluorophore.   Molecular 
Probes Prolong Gold (excellent quality) is our preference and you can seal 
with nail polish.  We have had the best luck with this particular mounting 
media even though there are others on the market, maybe you can Google to 
find more.  We have experienced fading of fluorophores with some of the 
other medias.  Also go to IHCWorld website and check out the discussion on 
autofluorescence/fluorescence - mounting medias are discussed there too.

Gayle M. Callis
Bozeman MT 59715

----- Original Message ----- 
From: "Igor Deyneko" 
Sent: Monday, January 28, 2008 10:26 PM
Subject: [Histonet] Aqueous Mounting Medium in In-Situ

> Dear Histonetters!
> I was wondering if anyone can help me with this question. I will be trying
> some In-Situ Hybridization and the protocol suggest covering the slides
> using thick Glycergel. i gave it a try today and get a lot of bubbles and
> overall it's hard to coverslip. I was wondering if anyone has ever used
> Aqueous Mounting Media to coverslip In-Situ slides? I used it for
> fluorescence and then sealed off the corners with clear nail polish.
> Thank you for any suggestions.
> Igor Deyneko
> Infinity Pharmaceuticals
> Cambridge, MA
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