Hi JR R,
You can go on line to the Biological Stains Commission
website and check out who else supplies Alcian Blue.
It won't give you the % of dye content, but will tell
you the various companies that have passed their
testing parameters. I for one like Anatechs' Alcian
Blue dye. They have always had a very consistant dye.
Hope this helps
--- JR R wrote:
> I have used Alcian Blue 8GX from Sigma for years
> with pretty good results. But I have had some
> variations in staining lately and noticed that the
> powder in the new lot isn't quite as dark as the
> previous lot. Finally, I noticed that Sigma only
> guarantees the product to be have a dye content of
> "at least" 50%.
> I would like to try to assay the dye content myself,
> so I could make my solutions to the sane strength
> every time. Sigma says they don't know hw, they
> have no idea what the molar extinction coefficient
> of the compound is, and they refuse to tell me the
> name of the lab that does the QC.
> The only thing I know is that it has a major
> absorbance peak at 610 nm.
> Oh, rumor says there was an aticle about it in
> Histochemie back in 1972.
> Any ideas?
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
> Need to know the score, the latest news, or you need
> your HotmailŪ-get your "fix".
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