Re: [Histonet] Acid Fast Bacteria/ Ziehl Neelsen

From:"Fred Underwood"

I know that storing in 10% NBF will.  I have a bottle full of positive lung that lit up when stained with AFB.  After a year or so in formalin, I get no staining what so ever.

>>> Rene J Buesa  1/25/2008 3:41 PM >>>
Theoretically it might. Remember that serous bacteria covering could be partially dissolved by EthOL. But try it, and let everybody know how it came about.
  René J

Lin Bustamante  wrote:
  Can long storage of wet tissue (over 6 months) in 70 % Etoh diminish the
reaction for positive infected tissue with Mycobacterium tuberculosis?
Thank you.

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab. Supervisor
Dept. of Veterinary Integrative Biosciences
Texas A&M University
College Station, TX 77843-4458

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