Dear Coral,
you may be decalcifying the specimen so that it will be hard to take a
good picture. Changing the buffer to PBS will improve the contrast
above background. If it isn't an issue with sectioning, you can
transition it to 100% ethanol for much better contrast. Averaging
several digital exposures will also increase signal:noise.
I can also do a nice overnight scan with our microCT
(http://ccri.uthscsa.edu/MicroCT.html) at no charge if you wish.
Sincerely,
Charles
Charles Keller, MD
Assistant Professor, Department of Cellular & Structural Biology
Adjunct Assistant Professor, Department of Pediatrics
Director, Small Animal Imaging Facility
Greehey Children's Cancer Research Institute
The University of Texas Health Science Center
8403 Floyd Curl Drive, Mail Code 7784
San Antonio, TX 78229-3900
210-562-9062 [office]
210-562-9014 [fax]
http://gccri.uthscsa.edu or
www.sarcomalab.org
kellerc2@uthscsa.edu
Postdoctoral Opportunities and
Undergraduate & Summer Internships:
http://ccri.uthscsa.edu/keller
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dickey,
Coral
Sent: Thursday, January 31, 2008 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xray contrast
Hello in Histoland,
I am trying to track the decalcification of some 42 day old mouse humeri
and femurs by xray. They have been in 10% EDTA for 36hrs and I just took
a film on KODAK BioMax MS film on a Faxitron xray machine. The bones
were exposed for 30 sec at 30kvp. I would like to increase the contrast
on the picture. Does anyone know how to do this.
Thanks in advance.
Coral Dickey
Histology Specialist I
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, Missouri 64110
Phone: 816-926-4305
e-mail: cad@stowers-institute.org
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