RE: [Histonet] PECAM-1 antibody

From:"Linke_Noelle"



Ahhhh....the infamous 'dead goat antibody' again raises it's ugly head!!

Ann....back in the day that antibody worked like a dream on rat and mouse, and then their goat died and it has not worked since....

If you ever find one that does work, you will be the queen of the Histonet!

DEAR VENDORS...PLEASE make us a CD31 antibody that works on rats and mice!

Noelle

NoŽlle Linke, MS, HTL(ASCP)QIHC
Allergan, Inc
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5568

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO
Sent: Friday, January 25, 2008 9:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PECAM-1 antibody

Hi, all

  I have been working on PECAM-1 antibody(Cat# sc-1506, goat 
ant-imouse ) from Santa Cruz on mouse,human and rat tissue, I 
tried a lot of methods(ABC-Elite method and HRP polyer method) and 
a lot of different AR, but could not let it work. The antibody 
stained everything including endothelial cell which I am really 
interested in. Is anybody here has experience on this antibody?

  Many tahanks,


Ann Dongtao Fu MD, Ph.D
Lab Manager
Molecular Pathology core
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610


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