RE: [Histonet] Kristensen's Sodium Formate/Formic acid recipe

From:"Rittman, Barry R"

340 gms dried sodium formate
1700 ml. w/v 90% formic acid
Distilled water to 10 liters.
Fix as usual
Rinse in running tap water  after formalin fixation.
Demineralize using agitation such as electronic stir bar.
(Difficult to over deminerlize with this formula).
Usually takes 3 to 5 times longer than 5% nitric acid.
Wash well in  running tap water .
Sorry don't have a reference as I am at home but have used this for several years and prefer this to all other demineralization methods except EDTA. Worked well with many IHC techniques.


From: on behalf of jstaruk
Sent: Sun 1/27/2008 6:04 PM
To: 'Histonet'
Subject: [Histonet] Kristensen's Sodium Formate/Formic acid recipe

Does anyone have the recipe and procedure for Kristensen's Sodium
Formate/Formic acid for demineralization of teeth?

Thank you


    Jim Staruk
Mass Histology Service

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