RE: [Histonet] AE1/AE3 cross reactivity

From:sheila adey

Our Dr.s have stopped ordering the AE1/AE3 ab aswell. For the same complaints. We use the Biogenex abs.
Sheila Adey HT MLTPort Huron HospitalMichigan> From:> To:;> Date: Thu, 17 Jan 2008 09:52:03 -0600> Subject: RE: [Histonet] AE1/AE3 cross reactivity> CC: > > We do not use AR for the AE1/AE3 on our Ventana system. We use Protease 1 for 4 minutes. I will check into the heat. Any other suggestions? We do the stains for Dr. Hessler.> Thanks,> Mighnon Lashus> > -----Original Message-----> From: [] On Behalf Of Sally Price> Sent: Wednesday, January 16, 2008 5:53 PM> To: Richard Hessler, M.D.;> Subject: RE: [Histonet] AE1/AE3 cross reactivity> > Dr. Hessler:> I have observed the phenomenon that you've described for other antibodies> more times than I can remember. Personally, I believe that it has to do> with the way the Ventana system sprays on the retrieval solution> and heats slides during antibody/detection incubation periods, which is too> aggressive. I think that an open system that doesn't heat slides or perform> AR isn't likely to produce these unexpalined reactions, but I digress. So,> how do you fix the problem? I say disable the on-line AR, and perform it in> a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could> consider using another pan-CK antibody, like Lu-5.> Good Luck,> Sally Price> > ----------------------------------------------------------------------------------------------> > Date: Wed, 16 Jan 2008 07:57:41 -0600> From: "Richard Hessler, M.D." > Subject: [Histonet] AE1/AE3 cross reactivity> To: ""> > "> I have used this antibody for years but now have issues. Previously it was> the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma> cells like CD138. It also stains a number of different sarcomas and> beautifully highlights reactive astrocytes. I had previously used Dako Ab> and autostainer with no problems. Now I have Ventanna and have noticed this> with both Ventana and Dako Abs used on that system.> > Any thoughts???> > Richard B Hessler, MD> Chief of Pathology> Erlanger Medical Center> Chattanooga, TN> _______________________________________________> Histonet mailing list>>> > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you> > _______________________________________________> Histonet mailing list>>

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