Dear Histonetters,
I am cryoprotecting mouse pancreas tissue (after fixation with
formalin) in 30% sucrose in PBS. After overnight incubation in
sucrose (at 4°C) some tissues sink, others do not. Is there a reason
(and remedy) for this ?
Thanks in advance,
Yves Heremans
Brussels Free University - VUB
Diabetes Research Center
Building D Level 2 Room D258
Laarbeeklaan 103
1090 Brussels, Belgium
Phone: +32 (0)2 477 44 73
www.betacelltherapy.org
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