[Histonet] re: Tissue not sinking in sucrose


After 4%PFA I like to cryoprotect in a sucrose gradient of 15% sucrose until the tissue sinks, then 25% to 30% sucrose until it sinks again. This gives me better results when frozen sectioning delicate tissues?like mouse brain, or embryos. I agree with Goef though, if it is fatty it will not sink.

Donna Emge HT-ASCP
Northwestern University
Feinburg School of Medicine
303 E. Superior, Lurie 7-220
Chicago, IL 60611
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