[Histonet] re: Tissue not sinking in sucrose

From:djemge@aol.com



After 4%PFA I like to cryoprotect in a sucrose gradient of 15% sucrose until the tissue sinks, then 25% to 30% sucrose until it sinks again. This gives me better results when frozen sectioning delicate tissues?like mouse brain, or embryos. I agree with Goef though, if it is fatty it will not sink.

Donna Emge HT-ASCP
Northwestern University
Feinburg School of Medicine
303 E. Superior, Lurie 7-220
Chicago, IL 60611
312-503-2036
d-emge@northwestern.edu
________________________________________________________________________
More new features than ever.  Check out the new AOL Mail ! - http://webmail.aol.com
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>