[Histonet] re: Nuclear Staining on Prostate Biopsies


This dark nuclei is often seen when tissue is left to dry for a few minutes before being fixed, or?if underfixed and?the alcohols on the processor fix the tissue. Since you are sure it is not dried or underfixed then it has to be the processing schedule. I agree with Renee. This sounds like a processing problem. Sounds like the overall schedule is too long for these biopsies. Especially if too long in paraffin.

Donna Emge HT-ASCP
Northwestern University
Feinburg School of Medicine
303 E. Superior, Lurie 7-220
Chicago, IL 60611
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