Your description sounds like you are trying to take the sections right from the microtome to the slide.
Try placing the sections on a room temperature waterbath containing weak ammonia. Put 4-5 drops of ammonium hydroxide in distilled water. Pick the sections up by dipping the slide in the water and bringing it up under the sections. This is opposite of picking ultra thin sections up on grids from the tiny waterbath on the knife on the ultra microtome.
Dry the sections before staining.
0.1% Toluidine blue is a good fast stain to see if you are where you want to be in the tissue before proceeding with other stains.
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
630 N. Broadway
Moore, OK 73160
Histonet mailing list