As always I will really appreciate if someone could give me some advice...
I need to stain peripheral blood with anti-GFP antibody and look with
immunofluorescence ("red" seeondary antibody).
My question is about the protocol:
Is it better blood smear, methanol fixation and antibody staining or is it
better to perform staining in the tube and then cytospin?
Could you refer me to a protocol?
thanks in advance
Berno Valeria, PhD
EMBL- Mouse Biology Unit
Campus A. Buzzati-Traverso
Via Ramarini, 32
00015, Monterotondo Scalo (RM)
Tel: +39 06 90091287
Fax: +39 06 90091406
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