I had this very same issue with Surgipath Hematoxylin a few months ago.
Jumped through hoops to try to fix the problem. Found a very effective and
fast solution after getting excellent responses on Histonet: I changed
brands of hematoxylin. Kept Surgipath reagents for the rest of our H&E
staining, but switched to a rebranded Hematoxylin from a local source (
Belair Instruments in New Jersey...dont know who the actual manufacturer of
the Hematoxylin is, but it works great). It seems more than a few other
people have had the exact same issue with Surgipath hematoxlyin. Alot of
people had switched to various different brands and fixed the problem. Its
a shame as we used the Surgipath brand hematoxylin for 12 years without
issue until this happened.
Ocean County Medical Lab
Brick, New Jersey
> Date: Tue, 22 Jan 2008 16:24:05 -0400
> From: "Greg Dobbin"
> Subject: [Histonet] Mucin containing cells too blue
> Content-Type: text/plain; charset=US-ASCII
> Hi Folks,
> I have been using the Surgipath Selectech reagents (H&E) for almost 3
> months now and I have not had a single complaint about staining...until
> now. Suddenly the mucin containing cells are too blue. My first
> thought was to make fresh Define (the Surgipath differentiator). This
> did not fix the problem. Next I replaced the hematoxylin with fresh
> (not supposed to have to change before 2500 slides, but changed at 1500
> slides to see if that would correct the problem). It has not.
> I was sure that fresh hematoxylin (and thererfore correct pH) would
> rectify the problem. What are the other usual suspects here? I should
> also mention our H&E's are done on the Leica Autostainer.
> I look forward to receiving your collective insight!
> Greg Dobbin, R.T.
> Chief Technologist, Histology Lab
> Dept. of Laboratory Medicine,
> Queen Elizabeth Hospital,
> P.O. Box 6600
> Charlottetown, PE C1A 8T5
> Phone: (902) 894-2337
> Fax: (902) 894-2385
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