It seemed to me that having to reanalyze a dye for its content was complicated and time consuming. As I cruised through past J of Histotechnology issues, I ran across a simple formula that may help speed things up.
X gram needed from new dye lot = 1 gram x % of dye in old dye lot
% dye content of new dye
We used to do this for the pyronin Y dye in a Methyl green pyronin method for DNA when dye lots were inconsistent and highly variable.
Gayle M. Callis
Bozeman MT 59715
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