It seemed to me that having to reanalyze a dye for its content was complicated and time consuming.   As I cruised through past J of Histotechnology issues, I ran across a simple formula that may help speed things up.  


X gram needed from new dye lot = 1 gram  x  % of dye in old dye lot  
                                                            % dye content of new dye


We used to do this for the pyronin Y dye in a Methyl green pyronin method for DNA when dye lots were inconsistent and highly variable.  

Good luck

Gayle M. Callis
HTL/TH/MT(ASCP)
Bozeman MT 59715
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