[Histonet] Basic Histology Questions

From:"Andrew Wang"


I am trying to stain IL-17 in mouse tissue.  I am having difficulty
and was wondering if anyone could guide me.  Also, I am lacking basic
histology knowledge and was hoping someone could clarify some issues
for me.

1. PBS vs TBS for wash (adding tween?)
2. PFA vs Formalin vs. Methanol vs. Acetone to fix
3. Saponin vs Triton for permeabilization?
4. Antigen unmasking: pepsin, citrate buffer?

I've been having trouble finding any information that would suggest a
specific reason for choosing any one of those variables, but i'm sure
there must be something out there?  Is there any logic behind any of
this "hoo ha?"

Ready to give up,

Andrew Wang MSIV
Department of Pathology
Tufts University Sackler School of Graduate Biomedical Sciences
University of Medicine and Dentistry of New Jersey
Robert Wood Johnson Medical School

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