I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system.
Richard B Hessler, MD
Chief of Pathology
Erlanger Medical Center
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