The prostatae are fixed in NBF at least for two days, then marked with black
and green ink and sliced up in 8-10 mm slides. 
We work with the big cassettes and molds. The VIP-protocol for the routine
specimen was just doubled in each station.
The thick slides tend to bend up in the center. So embedding should be done
with enough power to stick them on the ground.
Our routine microtomes are sliding microtomes, so the prostatae are also cut
on these and that works fine.
The HE protocol is the same as with routine specimen.
Hope this helps.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Metzger,
Kenneth
Gesendet: Dienstag, 22. Jänner 2008 21:03
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Whole Mount Prostates

Can anyone send or refer me to a processing protocol for whole mount
prostates? We haven't done them before and need some guidance. Any other
secrets or tricks on the cutting and staining would be greatly appreciated.
Ken

Ken Metzger HTL(ASCP)
Histology Supervisor
ARUP Laboratories
500 Chipeta way
Salt Lake City, UT 84108
801.583.2787 ext 3101


- ------------------------------------------------------------------
The information transmitted by this e-mail and any included
attachments are from ARUP Laboratories and are intended only for the
recipient. The information contained in this message is confidential
and may constitute inside or non-public information under
international, federal, or state securities laws, or protected health
information and is intended only for the use of the recipient.
Unauthorized forwarding, printing, copying, distributing, or use of
such information is strictly prohibited and may be unlawful. If you
are not the intended recipient, please promptly delete this e-mail
and notify the sender of the delivery error or you may call ARUP
Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1
(800) 522-2787 ext. 2100

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet