QDot website used to give protocols on how to work with these nanoparticles.
Also, in publications on QDots for estrogen receptors, you may be able to
dig out info from their methods and materials. Try PUBMED or Goggle Scholar
search.
Gayle M. Callis
HT/HTL/MT(ASCP)
Bozeman MT 59715
----- Original Message -----
From: "ratan choudhary"
To:
Sent: Thursday, January 10, 2008 11:33 AM
Subject: [Histonet] immunohistochemical detection of estrogen receptor
Hi,
I want to start detection of estrogen receptor using Qdots goat anti
rabbit secondary body. I am new to use qdots. Please suggest me dilution
rate and other crucial steps.
Thanks
Ratan
On 1/10/08, histonet-request@lists.utsouthwestern.edu <
histonet-request@lists.utsouthwestern.edu> wrote:
>
> Send Histonet mailing list submissions to
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>
> To subscribe or unsubscribe via the World Wide Web, visit
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>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. RE: Response to humor differences, etc. (Edwards, R.E.)
> 2. Agree to disagree (Webb, Dorothy L)
> 3. RE: Mart-1/Melan-A as a stain for melanoma sentinel nodes
> (Dawson, Glen)
> 4. Re: Who can perform Histology Duties (Rene J Buesa)
> 5. Mohs Tech position in Georgia (Shirley Powell)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 10 Jan 2008 17:09:07 -0000
> From: "Edwards, R.E."
> Subject: RE: [Histonet] Response to humor differences, etc.
> To: "Kathleen Roberts" ,
>
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
>
> "Emoticons", weren't they the baddies in an early episode of Dr.
> Who?
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen
> Roberts
> Sent: 10 January 2008 16:59
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] Response to humor differences, etc.
>
> Actually, in my experience, it isn't just a Histonet thing, it's an
> ONLINE thing. No matter what forum or list you're on, there is always
> misunderstanding, fights and flaming b/c two crucial things are missing
> in online forums: tone of voice and facial expressions. (Yes, even when
> those are present, misunderstandings, etc. still occur.) Emoticons
> don't even begin to cut it, and sometimes when a person is reading
> something, his/her mood, opinions, experience, etc. will "color" what's
> being read and thus influence the person's understanding of what was
> written, which the author never intended and can't control. I can't
> tell you how many times that has happened to me.
>
> Kathleen Roberts
>
> Dawson, Glen wrote:
>
> >Humor IS shared by both Americans and British, as is misinterpretation,
> misunderstanding and becoming indignant/offended over the strangest
> things.
> >
> >It isn't an Americans vs. the British thing, it's a histonet thing.
> Over-reactions are rampant and one should be aware that having a sense
> of humor should have a "Use at your own risk" sign when utilizing it on
> this forum. In every group there will always be a number of folks more
> interested in attacking what is being said by others rather than
> contributing something of their own.
> >
> >Each year this trend gets a bit worse & I find myself "replying to all"
> less and less. Posting to an individual usually causes less of a stir
> so that is the road I take much more these days.
> >
> >Just my .01 Euros,
> >
> >Glen Dawson
> >IHC Manager
> >Milwaukee, WI
> >
> >-----Original Message-----
> >From: histonet-bounces@lists.utsouthwestern.edu
> >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo
> >Rogerson
> >Sent: Thursday, January 10, 2008 8:44 AM
> >To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com;
> >histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org
> >Subject: RE: [Histonet] Response to On-Call pay
> >
> >
> >It's the humour Robyn, rarely travels well, I sometimes wonder if the
> >difference between Americans and the British is greater than that
> >between men and women.
> >
> >
> >Kemlo Rogerson
> >Pathology Manager
> >DD 01934 647057 or extension 3311
> >Mob 07749 754194; Pager 07659 597107;
> >Don't be afraid to take a big step when one is indicated. You can't
> >cross a chasm in two small jumps. --Buckminster Fuller
> >
> >This e-mail is confidential and privileged. If you are not the intended
>
> >recipient please accept my apologies; please do not disclose, copy or
> >distribute information in this e-mail or take any action in reliance on
>
> >its contents: to do so is strictly prohibited and may be unlawful.
> >Please inform me that this message has gone astray before deleting it.
> >Thank you for your co-operation
> >
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 10 Jan 2008 11:19:29 -0600
> From: "Webb, Dorothy L"
> Subject: [Histonet] Agree to disagree
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> <0E394B648E5284478A6CCB78E5AFDA27056353B9@hpes1.HealthPartners.int>
> Content-Type: text/plain; charset="us-ascii"
>
> I agree with Glen inasmuch as I do not do a lot of "post" responses due
> to being attacked! Constructive criticism is great and offering gentle
> advice is even better, but not "putting one down" because they do not
> know as much as someone else may or have a different viewpoint!! I
> still LOVE the histonet and appreciate all of the great wisdom instilled
> in me by those of you who keep responding to my inquiries and others!!
>
> Dorothy Webb, HT (ASCP)
> Histology Technical Supervisor
> Regions Hospital, Pathology Department
> 640 Jackson Street, Saint Paul, MN 55101-2595
> Phone: 651-254-2962
> Fax: 651-254-2741
> Regions Hospital is part of the HealthPartners family of care
> ________________________________________
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to whom they are
> addressed. If you are not the intended recipient or the individual
> responsible for delivering the e-mail to the intended recipient, please be
> advised that you have received this e-mail in error and that any use,
> dissemination, forwarding, printing, or copying of this e-mail is strictly
> prohibited.
>
> If you have received this e-mail in error, please immediately notify the
> HealthPartners Support Center by telephone at (952) 967-6600. You will be
> reimbursed for reasonable costs incurred in notifying us.
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 10 Jan 2008 11:23:52 -0600
> From: "Dawson, Glen"
> Subject: RE: [Histonet] Mart-1/Melan-A as a stain for melanoma
> sentinel nodes
> To: "Barnhart, Tammy" , "Histonet
> \(E-mail\)"
>
> Message-ID:
> <
> B3D65550856D0146B900D401EE313D4B01274146@dynams.dynacaremilwaukee.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Tammy,
>
> I use a cocktail of Mart-1, Melan-A & Tyrosinase that we named the MCW
> (Medical College of Wisconsin) Melanoma Cocktail. If you google it you
> should find our paper online and I do believe that my Pathologist
> addresses
> some Mast cell staining issues in it. The triple antibody is excellent as
> a
> screener as it covers more epitopes than any one of the three does
> individually. Suggested staining methods are in the paper as well.
>
> Best of Luck,
> Glen Dawson
> IHC Manager
> Milwaukee, WI
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Barnhart,
> Tammy
> Sent: Thursday, January 10, 2008 10:51 AM
> To: Histonet (E-mail)
> Subject: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel
> nodes
>
>
> We are having some debate about which marker to use for our melanoma
> sentinel node screens. We have been using Mart-1, but recently had a case
> where we think mast cells were stained. We can find no literature that
> indicates that Mart-1 will stain mast cells. We use Lab Vision's
> Mart-1/Melan-a AB-3 (clone M27C10+M2-9e3). We ran a Mast Cell tryptase to
> see what stained, and all the cells that stained with the Mart-1 stained
> plus a bunch of others. So if the Mart-1 is staining mast cells, it is
> only
> staining a subset of them. We really think that these are mast cells and
> not melanoma, but would like to see some literature to support this. So
> two
> questions: 1) What are people using as a screening antibody and 2) Does
> anyone have a reference that indicates that this antibody will stain mast
> cells. Thanks in advance for your help.
>
> Tammy Barnhart, BS, HTL(ASCP)
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> Bismarck, ND
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 10 Jan 2008 09:41:38 -0800 (PST)
> From: Rene J Buesa
> Subject: Re: [Histonet] Who can perform Histology Duties
> To: Terri Braud ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <463239.48520.qm@web61219.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> In the same way a "well trained woo-doo priest" can perform surgery? What
> about the knowledge background? Irrelevant?
> René J.
>
> Terri Braud wrote:
>
>
>
>
> ---------------------------------
> Looking for last minute shopping deals? Find them fast with Yahoo!
> Search.
>
> ------------------------------
>
> Message: 5
> Date: Thu, 10 Jan 2008 12:52:13 -0500
> From: Shirley Powell
> Subject: [Histonet] Mohs Tech position in Georgia
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <01MPXG4PEYQE003MTI@Macon2.Mercer.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Please Do Not reply to me. I am posting this for a friend.
> Please contact the Dr. Lane or Renee Blanton at the numbers below.
>
> Needed immediately
> Certified Mohs histotech
>
> Exciting new Mohs surgery practice in Columbus, Georgia
>
> Part-time or full-time possible
>
> Competitive pay
> Excellent benefits
>
> Contact: Joshua Lane, M.D. at 706.573.6545 or Renee Blanton at
> 706.322.1717
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 50, Issue 15
> ****************************************
>
--
***********************************************
Ratan Kumar Choudhary
Graduate Research Assistant
Bovine Functional Genomics Lab
Room 14
Bldg 200 BARC-East
Powder Mill Road
Beltsville, Maryland, 20705
USA
Phone: 301-504-8342 (O)
301-931-8730 (R)
Fax: (301) 504-8414
http://www.drratankumar.wetpaint.com
***********************************************
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