Re: [Histonet] Deterioration rate of fluors used in IF staining

From:Rene J Buesa



Sally:
  The IF reagents I used (FITC conjugated Ig) I used to aliquot them and keep them at -80║C until needed. Once the slide was stained I always tried to read as soon as possible. Otherwise I kept the slides on a refrigerator for, the most 3 days, and after that the fluorescence faded.
  There are coverslipping solutions that reduce the fading rate also. The best way of keeping fluorescent reagents is in a refrigerator (where they are also protected from light).
  RenÚ J.
  

Sally Price  wrote:
  
 

       
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