Re: [Histonet] Deterioration rate of fluors used in IF staining

From:Rene J Buesa



Sally:
  The IF reagents I used (FITC conjugated Ig) I used to aliquot them and keep them at -80ºC until needed. Once the slide was stained I always tried to read as soon as possible. Otherwise I kept the slides on a refrigerator for, the most 3 days, and after that the fluorescence faded.
  There are coverslipping solutions that reduce the fading rate also. The best way of keeping fluorescent reagents is in a refrigerator (where they are also protected from light).
  René J.
  

Sally Price  wrote:
  
 

       
---------------------------------
Looking for last minute shopping deals?  Find them fast with Yahoo! Search.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>