Re: [Histonet] CD11c on paraffin?

From:"Gayle Callis"



David,

We use BDPharmingen ala Invitrogen rat anti mouse CD11c on murine tissue, 
but only on frozen sections for both immunhistochemistry and 
immunofluorescence.   This antibody proved to be far superior to another we 
were using and you can access the BD product data sheet online to see what 
they say about its use with FFPE.

Have you looked in Histonet Archives as this question has been asked before.

Gayle M. Callis
HT/HTL/MT(ASCP)
Bozeman MT

----- Original Message ----- 
From: "Kinsley, David" 
To: 
Sent: Tuesday, January 08, 2008 4:50 PM
Subject: [Histonet] CD11c on paraffin?


Hi,

Does anyone know of a CD11c antibody that works on FFPE tissue sections?
I'm looking for antibodies that work on either human or mouse tissues.
My samples are fixed in formalin for 24 hours then transferred to 70%
ETOH and processed into paraffin.

Thanks in advance

Dave
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