Hi:

We use the Davidson (sp) tissue marking dye on all our prostate biopsies and do not have any problems with our immunos.  We do not use the red dye though, because we are afraid it might be confused with AMACR staining on the PIN4 cocktail.

Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory
ph:  708-486-0076
fax: 708-486-0080



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ford, Judi
Sent: Thu 1/10/2008 5:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue marking dyes and IHC
 
Hi all,

I was just curious to find out if tissue marked with either hematoxylin
and/or eosin, or tissue marking dyes, can that affect future IHC and/or
immunofluorescent staining? We have a situation where a right and left
piece of tissue will be embedded on one block and we want to distinguish
the two pieces. They are very small DRG's which can get lost once
embedded. One thought was to use dyes to mark them, but I was concerned
about the possibility of interference with immuno staining.

I'd love to hear your thoughts.

Thanks soooo much! 

Judi Ford, BA, HTL(ASCP)
Research Associate III
Roche Palo Alto
3431 Hillview Ave
Palo Alto, CA 94304


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