I have had very good luck with IHC on formalin-fixed, paraffin embedded tissue that was previously stained with eosin. The eosin comes out when I deparaffinize and rehydrate the slides.
University of Washington
Department of Pathology
> Date: Thu, 10 Jan 2008 15:34:33 -0800> From: firstname.lastname@example.org> To: email@example.com; firstname.lastname@example.org> Subject: RE: [Histonet] Tissue marking dyes and IHC> CC: > > Hi Judi, I have not tested the hypothesis, but I worked for a> pathologist who would not let us use anything but black India ink on any> tissue that was to be tested with ER/PR IHC because plant dyes may> contain their own estrogens. Tom Truscott USDA> > -----Original Message-----> From: email@example.com> [mailto:firstname.lastname@example.org] On Behalf Of Ford,> Judi> Sent: Thursday, January 10, 2008 3:19 PM> To: email@example.com> Subject: [Histonet] Tissue marking dyes and IHC> > Hi all,> > I was just curious to find out if tissue marked with either hematoxylin> and/or eosin, or tissue marking dyes, can that affect future IHC and/or> immunofluorescent staining? We have a situation where a right and left> piece of tissue will be embedded on one block and we want to distinguish> the two pieces. They are very small DRG's which can get lost once> embedded. One thought was to use dyes to mark them, but I was concerned> about the possibility of interference with immuno staining.> > I'd love to hear your thoughts.> > Thanks soooo much! > > Judi Ford, BA, HTL(ASCP)> Research Associate III> Roche Palo Alto> 3431 Hillview Ave> Palo Alto, CA 94304> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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