RE: [Histonet] Counterstain on Ventana stainers

From:"Tarango, Mark"

I've used Richard-Allen's 7211 hematoxylin for a minute, and then their
clarifier 2 for minute, and finally blue with buffer.  That's three
steps, but it looked the best compared to other things I've tried.

Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663


-----Original Message-----
[] On Behalf Of Drew
Sally A.
Sent: Thursday, January 10, 2008 8:30 AM
To: Histonet
Subject: [Histonet] Counterstain on Ventana stainers

Is anyone using a non-Ventana hematoxylin counterstain on their
immunostainers?  If so, what works best for you in terms of intensity
and stability.  Just looking at a bit of cost-cutting...
Thank you for your time!

Sally Ann Drew, MT(ASCP)
IHC/ISH Laboratory
University of Wisconsin Hosp. & Clinics
Madison, WI 53792

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