[Histonet] H & E stain question


We are continually having a problem with our H & E stain.  We currently
use Gill III Hematoxlyin on a staining machine with a running water
bath.  Our pathologists do not like to have the pH of the Heme higher
than 2.5 because it makes the small biopsy sections too dark and the
mucin appears blue.  My question to all of you is, has any of you
experienced this issue and if so, how did you go about getting it fixed?
I had suggested switching to a different heme such as Harris and maybe
switching our running water bath to DI water.  We have significant
flooding in our area and there will be more bleach in the city water
supply.  Does any of this sound feasible?  Help please.  Thanks.


Sharon Davis-Devine, CT (ASCP)

Cytology Supervisor

Carle Clinic

602 West University

Urbana, Illinois 61801

Phone:  217-383-3572

Email:  sharon.davis-devine@carle.com


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