re: [Histonet] Markers of oxidative damage

From:bhewlett@cogeco.ca@cogeco.ca

> Hi Sony,

8-OHdG and 8-OG can be demonstrated by means of their biotin-like reactivity.
We have seen this in a number of cases when it appeared as a specific background, in both the patient test and negative
reagent control when staining for assorted antigens by IHC.

See following reference;
Struthers L, Patel R, Clark J, Thomas S.
Direct detection of 8-oxodeoxyguanosine and 8-oxoguanine
by avidin and its analogues.
Analytical Biochemistry 1998; 255: 20-31.

Regards,

bryan


> Hi all,
>
> I am trying to get information on markers of oxidative damage (8-OHdG, 4-HNE
> or any others that might work). I have some formalin fixed paraffin embedded
> mouse kidneys and I need to assess the oxidative damage to the tissue via
> immunohistochemistry.
> If anyone has used such markers and has any protocols or suggestions I would
> highly appreciate it.
>
> Many thanks,
> Sony Prasad.
> Research Student,
> University of Sheffield.
>
> _________________________________________________________________
> Over 200000 Jobs @ naukri.com ! Choose The Best One
> http://naukri.com/tieups/tieups.php?othersrcp=4358
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>