Re: [Histonet] project on microwave

From:Phil McArdle

Hello:

A microwave vendor weighs in:

EBS' position is that domestic microwaves not be used for lab work, but 
then, we're in the position of manufacturing lab microwaves. :-) That 
said, in this case I can understand the temptation to do so, given the 
academic context and undoubtedly inherent budget constraints. I would be 
careful, however: of all the operations lab microwaves can be used for, 
fixation and tissue processing are the most demanding and require the 
tightest temperature control.

Besides the issue of a temperature probe (most domestic microwaves don't 
have one) there is the issue of magnetron cycle time; domestic 
microwaves' cycle times are typically too long to allow the temperature 
control required for small, delicate histological samples. The chances 
of cooking your tissue are great.

I'd explore other options: local locations with laboratory microwaves 
who might let you into their lab. Especially for fixation and 
processing, a true laboratory microwave is the surest path to success.

Phil McArdle
-- 

Microwave Product Manager

Energy Beam Sciences, Inc.
29-B Kripes Rd.
East Granby, CT 06026

Tel:  800.992.9037 x 341
Fax: 860.653.0422

pmcardle@ebsciences.com
www.ebsciences.com

You must be the change you want to see in the world.
- Mahatma Gandhi

NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you.



rosadel holgado wrote:
> Hi!
>    
>   I am a newbie here and I wonder if it's appropriate to be asking questions here about my project. I am planning to do a project for my MSc soon and I was thinking of doing something about microwave fixation and microwave assisted processing.  Does anyone know of a protocol I can cite for using a domestic microwave for the fixation? 
>    
>   Thanks!
>    
>   Rosadel Salita
>   Histology
>   Surrey, England
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>