William

Can you tell me what type of tissue are you cutting? Do you do your own
tissue processing or is sent to another lab? What paraffin do you use? What
blade do you use, disposable or steel.

Sometimes brittleness comes from taking the water out of the tissue. That
can be helped by improving your processing schedule.

Let me know the answers when you can.

Best Regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot@leica-microsystems.com
www.leica-microsystems.com


                                                                           
             William Ares                                                  
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             lists.utsouthwest                                             
             ern.edu                                               Subject 
                                       [Histonet] paraffin sectioning      
                                       troubleshooting                     
             01/19/2007 10:11                                              
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           



As a newbie to paraffin sectioning on a paraffin microtome, I've been
plagued with differential results since starting.  My current issue is that
as they're cut, my sections are rolling back up on themselves instead of
coming off the blade with the appearance of a venetian blind (as they were
when the sections were looking good).  As they appear brittle, I've tried
putting the blocks in a humid environment to try to rehydrate them a
little, but to no avail.  Anyone have any good troubleshooting tips for me?
Thanks in advance.

William Ares
Research Technician
Laboratory of Molecular and Developmental Neuroscience
Weill Medical College of Cornell University
Tel: (212) 746 5056
Email: wia2005@med.cornell.edu


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