Re: [Histonet] Trying to get consensus - Frozen section immediate post fixation or air dry

From:Gayle Callis


How you treat a frozen section is going to depend on what you are planning 
to do with the frozen section.  If you can be more specific that will help.

Do you want to immunohistochemical staining on a snap frozen tissue?

Do you want to fix and then store these frozen sections for 

Do you want to store an unfixed frozen section for future 
immunohistochemical staining?

  Do you want to do a rapid H&E for diagnostic purposes?

We do any one of the above depending on a particular staining procedure 

At 11:04 AM 1/18/2007, you wrote:
>Hi Histonet
>I have seen two basic camps regarding the treatment of frozen sections
>once the section hits the slide - One camp fixes immediately and one
>camp leaves the section to air dry, sometimes overnight.  I realize that
>there is likely not a "right" answer, but are there advantages to one
>process method over another? I'm in the midst of trying out both
>processes and evaluating myself, but in the meantime I'd like to hear
>feedback from the community.

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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