Re: [Histonet] Re: Goldner's Trichrome Staining

From:"Bryan Hewlett"

Hi Bob,

Interesting background.

All of these dyes are currently available!
The problem in obtaining them may be the use of various common names to 
describe them.
The use of  the listed name and the CI # and CI name indicates a specific 
dye, synonyms may be confusing since they can be shared with other 
non-related dyes.
The following is from the latest edition of Conn's Biological Stains.

Acid fuchsine, CI 42685, CI Acid violet 19, synonym: acid magenta.
Commonly available.

Ponceau de xylidine is still available from a number of suppliers.
Listed as Ponceau 2R, CI 16150, CI Acid red 26, synonym: ponceau de 
The US Sigma catalogue # is P2395.

Orange G, CI 16230, CI Acid orange 10, synonym: wool orange 2G.
Commonly available.

Light green SF, CI 42095, CI Acid green 5, synonym: light green SF 
Commonly available.

Best regards,


----- Original Message ----- 
Sent: Wednesday, January 24, 2007 3:29 PM
Subject: [Histonet] Re: Goldner's Trichrome Staining

> Tiffany Pitts (University of Washington state) is trying to reconstruct a
> "Goldner trichrome stain", and Gudrun Lang (Analytikerin in Linz, Germany)
> assists. (Some history may help - see below.)- I've reproduced their 
> formulas,
> standardizing English spellings for the convenience of the Googler:
> Deplasticize, rehydrate, wash
> stain nuclei in Weigert's hematoxylin
> wash, 1% HCl, wash
> blue in saturated lithium carbonate
> wash
> stain in "Goldner's Trichrome Stain" reagent (?!?!?)
> wash for 1 min, dehydrate and coverslip
> It is the "Goldner's Trichrome Stain" reagent that I have run out of and 
> when
> I looked up my former colleague's notes she has "See Julie" instead of a
> recipe. Well, Julie is no longer with us either and I am running out of 
> places to
> look!
> nuclei-staining with Weigert hematoxylin
> Staining in a mixture of acid fuchsin (or fuchsine) and ponceau de 
> xylidine
> (0.2 g ponceau de xylidine and 0.1 g acid fuchsin in 300 mL distilled 
> water
> with
> 0.6 ml 100% [glacial] acetic acid)
> Rinse in 1% acetic acid
> Stain in PMA-Orange G solution (few minutes)
> (3-5g phosphomolybdic acid and 2 g Orange G in 100 mL distilled water)
> Rinse in 1% acetic acid
> Counterstain with Light Green SF (0.1% 100 mL plus 0.2 mL 100% acetic 
> acid)
> 5 min 1% acetic acid
> Dehydrate, clear and mount
> *****************
> I probably have more information, but I'm away from my books this week. 
> You
> may have difficulty in obtaining some of these dyes, particularly ponceau 
> de
> xylidine (probably the same as ponceau red).
> Pierre Masson in Montreal (1920's - 1930's) is supposed to have developed
> many variants of his trichrome stain, and there is no one "Masson stain".
> Goldner's variant, which Gudrun Lang describes, was taken up by Chandler 
> Foot at Co
> rnell Medical Center/New York Hospital in the 1930's as a general 
> oversight
> stain in place of H & E, and the stain was sometimes called the 
> Goldner-Foot
> stain. Chandler Foot, one of the founders of American surgical pathology, 
> died in
> 1948. Most of his slides were destroyed in a management disaster around 
> 1960,
> but some still survived in teaching collections when I was a resident 
> there in
> 1968. - George Papanicolaou, at Cornell around 1940, probably used this 
> stain
> as the basis of the still-used "Pap stain", though certain historical 
> proof of
> this point is probably lacking (Gary Gill, do you know?).
> I would suggest a modern green trichrome stain - they're available
> commercially, though most pathologists have abandoned them because they 
> aren't good for
> liver biopsies. I'd advise separating the green dye and the orange G, but 
> in
> order to reproduce your present stain, you may need to seek out or prepare 
> a
> stain mixture that combines them.
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
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