Re: [Histonet] Processing debate

From:Rene J Buesa

Fear the word "random", in histology is almost synonym with "caos".There is absolutely no logic or benefit from placing the cassettes at random.
  If you use a cassetting log the sequencial and orderly manner of placing the cassettes following the log order is the way of doing things.
  You can always know where your cassettes are and at the embedding step you can follow the same sequency and easily determine if all the cassettes were processed and embeded.
  It is absolutely illogical to place anything at random (unless you are selecting something for an experimental design that requires that type of selection!).
  The only "logical" explanation to your initial question is that it stems from the pathologist's lazyness that prefers to throw the cassettes in the container and letting the histotech to try to organize what should not have been disorganized in the first place.
René J. wrote:
  We presently process with a Tissue Tek Vip 2000. Our pathologist cut
the tissue in. the debate is this ... one side says that the cassettes
must be placed in a container of formalin to float at random before being
placed into the Vip 2000 basket.
The other side of the debate says that the tissue cassettes can be
placed in order directly into the basket that is submerged in formalin.
Can the group shine any light on this so far civil debate :)

Gary Martin
El Dorado Pathology

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